Publications

This page will be a curated list of significant publications that have used the proteome array platform. This will NOT be a complete list of papers, but is instead intended to showcase examples of different types of data analysis, graphical output, study design or other innovations related to the array platform.

2015

Dotsey EY, Gorlani A, Ingale S, Achenbach CJ, Forthal DN, Felgner PL, Gach JS.
A High Throughput Protein Microarray Approach to Classify HIV Monoclonal Antibodies and Variant Antigens. PLoS One. 2015 May 4;10(5):e0125581. Link

  • significant because it uses the proteome array to calculate half maximal effective concentrations (EC50) and affinity constants by titrating antibody concentration on the chip by serial dilution.

Dent AE, Nakajima R, Liang L, Baum E, Moormann AM, Sumba PO, Vulule J, Babineau D, Randall A, Davies DH, Felgner PL, Kazura JW. Plasmodium falciparum Protein Microarray Antibody Profiles Correlate With Protection From Symptomatic Malaria in Kenya. J Infect Dis. 2015 Apr 15. pii: jiv224. Link

  • significant because of the simple but powerful study design to identify protective antigens in naturally acquired immunity. Serum samples were taken on day 0 from a cohort of Kenyan individuals, who were then cleared of any parasites using antimalarials. The cohort was then followed for 12 weeks to identify those that became re-infected. Of these, some were unprotected from malaria, i.e., developed typical symptoms (fever, headache etc), while others felt perfectly healthy. By using the arrays, antibodies to a number of antigens that were significantly elevated on day 0 in the protected individuals (i.e., those that went on to develop asymptomatic infections) were identified. These may mediate protection against malaria symptoms and represent candidate vaccine antigens.
  • also significant because it uses proteome array data from a cross sectional survey of a Kenyan population (i.e., spanning all ages) to calculate annual seroconversion rates (SCRs) for each antigen. Antibodies to some antigens are acquired early in life while others are acquired more gradually. Since it takes several years of exposure to parasites to acquire natural immunity to malaria disease, defining SCRs will help understand the age-dependent acquisition of natural immunity.